ABSTRACT
Digital microfluidic biochips are being utilized in many areas of biochemistry and biomedical sciences. Since cross-contamination between droplets of different biomolecules can lead to erroneous outcomes for bioassays, it is essential to avoid cross-contamination during droplet routing. We propose a wash-operation synchronization method to manipulate wash droplets to clean the residue that is left behind by sample and reagent droplets. We also synchronize wash-droplet routing with sample/reagent droplet-routing steps by controlling the arrival order of droplets at cross-contamination sites. The proposed method minimizes droplet-routing time without cross-contamination, and it is especially effective for tight chip-area constraints. A real-life application is used for evaluation.
- R. B. Fair et al., "Chemical and biological applications of digital-microfluidic devices", IEEE Design & Test of Computers, vol. 24, pp. 10--24, 2007. Google ScholarDigital Library
- K. Chakrabarty and F. Su, Digital Microfluidic Biochips: Synthesis, Testing, and Reconfiguration Techniques, CRC Press, Boca Raton, FL, 2006.Google ScholarCross Ref
- Advanced Liquid Logic, http://www.liquid-logic.com.Google Scholar
- J. Berthier and P. Silberzan, Microfluidics for Biotechnology, Artech House, 2005.Google Scholar
- K. F. Bohringer, "Modeling and controlling parallel tasks in droplet-based microfluidic systems", IEEE Trans. CAD, vol. 25, pp. 334--344, 2006. Google ScholarDigital Library
- M. Campas and I. Katakis, "DNA biochip arraying, detection and amplification strategies", Trends in Analytical Chemistry, vol. 23, pp. 49--62, 2003.Google ScholarCross Ref
- A. B. Fuchs et al., "Electronic sorting and recovery of single live cells from microlitre sized samples", Lab on a Chip, vol. 6, pp. 121--126, 2006.Google ScholarCross Ref
- E. J. Griffith, S. Akella, and M. K. Goldberg, "Performance characterization of a reconfigurable planar-array digital microfluidic system", IEEE Trans. CAD, vol. 25, pp. 345--357, 2006. Google ScholarDigital Library
- V. Hlady, R. A. Wagenen and J. D. Andrade, Surface and Interfacial Aspects of Biomedical Polymers: Protein Adsorption; Andrade, J. D. Ed.; Plenum Press: New York, 1985, 2, pp. 81.Google Scholar
- M. Cho and D. Z. Pan, "A high-performance droplet routing algorithm for digital microfluidic biochips", IEEE Trans. CAD, vol. 27, pp. 1714--1724, 2008. Google ScholarDigital Library
- H. Ren et al., "Design and testing of an interpolating mixing architecture for electrowetting-based droplet-on-chip chemical dilution", Transducers, 2003.Google Scholar
- K. Sermon et al., "Preimplantation diagnosis for Huntington's disease (HD): clinical application and analysis of the HD expansion in affected embryos", Prenatal Diagnosis, vol. 18, pp. 1427--1436, 1999.Google ScholarCross Ref
- V. Srinivasan et al., "Protein stamping for MALDI mass spectrometry using an electrowetting-based microfluidic platform", Proc. SPIE, vol. 5591, 2004.Google Scholar
- F. Su, W. Hwang, and K. Chakrabarty, "Droplet routing in the synthesis of digital microfluidic biochips", Proc. DATE, pp. 323--328, 2006. Google ScholarDigital Library
- T. Xu, K. Chakrabarty and V. K. Pamula, "Defect-tolerant design and optimization of a digital microfluidic biochip for protein crystallization", IEEE Trans. Comput.-Aided Design Integr. Circuits Syst., vol. 29, pp. 552--565, 2010. Google ScholarDigital Library
- T.-W Huang, C.-H. Lin and T.-Y. Ho, "A contamination aware droplet routing algorithm for digital microfluidic biochips", Proc. ICCAD, 2009.Google Scholar
- Y. Zhao and K. Chakrabarty, "Cross-contamination avoidance for droplet routing in digital microfluidic biochips", Proc. DATE, 2009. Google ScholarDigital Library
- P.-H. Yuh et al., "BioRoute: A network flow based routing algorithm for the synthesis of digital microfluidic biochips", IEEE Trans. CAD, vol. 27, pp. 1928--1941, 2008. Google ScholarDigital Library
- P.-H. Yuh et al., "A progressive-ILP based routing algorithm for cross-referencing biochips", Proc. DAC, pp. 284--289, 2008. Google ScholarDigital Library
Index Terms
- Synchronization of washing operations with droplet routing for cross-contamination avoidance in digital microfluidic biochips
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